• Mamay Mamay STIKes Karsa Husada Garut
  • Yadi Apriyadi RSUD dr.Slamet,Garut
  • Astari Nurisani STIKes Karsa Husada Garut
  • Dian Hardianti STIKes Karsa Husada Garut
Keywords: Breast cancer, crystal violet, hematoxylin, staining


Microscopic examination of cancer development can be identified through histopathological examination. Hematoxylin–eosin (HE) is the gold standard for staining method. This processing can be obstructed if hematoxylin reagent out of stock in the laboratory. Other reagents are needed that can replace the use of hematoxylin, one of which is crystal violet. This study aims to evaluate the use of crystal violet as a nuclei stain in breast cancer preparations. The research sample consisted of 15 breast cancer preparations taken at the sample bank in the anatomical pathology laboratory of RSUD Dr. Slamet Garut. The results showed that from 15 preparations, it was observed that 4 preparations (27%) stained worth and 11 preparations (73%) stained poorly. Based on the research results, crystal violet crystal violet has a low core coloring ability in breast cancer preparations. From the results of this study, it is recommended to modify the crystal violet reagent by paying attention to the acidity of pH and concentration of crystal violet.


Al-Khikani F, Ayit A. (2022). The Antibacterial Action of Safranin and Gentian Violet. Rambam Maimonides Med 31;13(3):e0018. doi: 10.5041/RMMJ.10475. PMID: 35921487; PMCID: PMC9345769.
Alturkistani HA, Tashkand FM, Saleh ZMM. (2016). Histological Stains: A Literature Review and Case Study. Glob J Health Sci 8;72–79.
Bahrami A, Amiri MJ, Bagheri F. (2020). Optimization of crystal violet adsorption by chemically modified potato starch using response surface methodology. Pollution, 6(1): 159-170.
Bendzinski ECM, Chu K, Johnson JI, Brous M, Copeland K, Bolon B. (2020). Optical density-based image analysis method for the evaluation of hematoxylin and eosin staining precision. J Histotechnology;29–37.
Chapman CM. (2019). Troubleshooting in the histology laboratory. J Histotechnology 42;137–149
Gurina TS, Simms L. Histology, Staining. (2022). In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishinvailable from: https://www.ncbi.nlm.nih.gov/books/NBK557663/
Indonesian Ministry of Health. (2022). Most Common Breast Cancer in Indonesia, Ministry of Health Targets Equalization of Health Services. Jakarta.
Kadoo P, Dandekar R, Kulkarni M, Mahajan A, Kumawat R, Parate N. (2017). Correlation of mitosis
obtainned by using 1% crystal violet stain with Ki67LI in histological grades of oral squamous cell carcinoma. J Oral Biol Craniofacial Res. 8;234–240
Kiuchi K. (2016). Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections. Clin Ophthalmol 10;1971–1975.
Levine M.S,Holland A.J (2018) The impact of mitotic errors on cell proliferation and 673 tumorigenesis. Genes Dev. 32, 620-638.
Mahmoud H. Abu Elella, Magdy W. Sabaa, Eman Abd ElHafeez, Riham R. Mohamed. (2019). Crystal Violet Dye Removal Using Crosslinked Grafted Xanthan Gum. S0141-8130(19)31902-6.
Meyerholz DK, Beck AP. (2018). Principles and approaches for reproducible scoring of tissue stains in research. Laboratory Investigation, Volume 98, Issue 7:844-855,
Pathak, R., Jha, A., Neupane, P., Chalise, S., & Basnyat, A. (2016). Histopathological evaluation of carcinoma of breast. Journal of Pathology of Nepal, 6(11), 922–927.
Rahmawati T, Arpiyadi Y, Mamay. (2020) Utilization of 1% of Methylene Blue in Staining Histopathological Preparations at Anatomic Pathology Laboratory. Ina J Med Lab Sci Tech 2(1): 93-100
Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bry F. (2020). Global cancer statistics: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021: 71: 209-249.
Tandon A, Singh NN. Brave, VR Sreedhar G. (2016) . Image analysis assisted study of mitotic figures in oral epithelial dysplasia and squamous cel carcinoma using differential stains. J Oral Biol Craniofacial Res 6;18–23
Veuthey, T., Georgina Herrera, G, Dodero, V.I. (2014) .Dyes and stains: From molecular structure tohistological application. Front in Biosci;19: 91–112.
How to Cite
Mamay, M., Apriyadi, Y., Nurisani, A., & Hardianti, D. (2023). UTILIZATION CRYSTAL VIOLET AS NUCLEI DYE IN HISTOPATHOLOGY OF BREAST CANCER. Jurnal Medika Cendikia, 10(2), 97-102. https://doi.org/10.33482/jmc.v10i2.226